Recombinant protein production for structural and functional studies

Biomedical research relies heavily on purified recombinant proteins, playing a crucial role in a wide spectrum of applications.

Their homogeneous nature allows for detailed reconstruction of their three-dimensional structures using techniques such as X-ray, cryoEM, and NMR, facilitating a deeper understanding of protein folding, conformational changes, molecular architectures, enabling studies of their function.

Additionally, these proteins are essential in investigating various interactions, such as protein-protein, protein-ligand, or protein-nucleic acid, and serve as critical tools in the field of therapeutics and diagnostics.

To guarantee the reliability of experimental data in these studies a good quality of recombinant protein is essential.

In this presentation the process of protein production is described, giving an overview of critical steps from construct design through expression, purification and quality control.

Speaker: Marta Semrau, RIT (Area Science Park)